Coding

Part:BBa_K4220007:Design

Designed by: Xiaoya Liu   Group: iGEM22_NEU_CHINA   (2022-09-25)


P2Y2-P2A-Galpha


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1159
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1095
    Illegal AgeI site found at 389
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 570
    Illegal SapI site found at 615


Design Notes

In order to improve the capability for P2Y2 to be responsiveness to extracellular ATP (eATP), substitutions (K240N and F307S) will be applied.


Source

P2Y2 (GPCR) is from human genomic sequence. Galpha is a chimeric protein: Gpa1 is from yeast and 5 C-terminal amino acids are from human Galphai3.

References